Device

Part:BBa_K2080004

Designed by: Haojian Li   Group: iGEM16_SCUT-China_B   (2016-09-24)


bcl2-sgRNA1

This guide RNA is a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2.

Usage and Biology

This guide RNA is for dCas9-KRAB targeting the bcl-2 promoter, 18 bp upstream the bcl-2 promoter.

Charaterization

This guide RNA is for dCas9-KRAB targeting the bcl-2 promoter. The transcription of bcl-2 is downregulated by the dCas9-KRAB-sgRNA complex. The transcription level of Bcl-2 was detected by real-time PCR(Fig.1).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None